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Synthetic mRNA

Name: Synthetic mRNA
Brand: Humana Press Inc.
SKU: 9781493936236
Price: 711.37 PLN
Availability: InStock

Production, Introduction Into Cells, and Physiological Consequences

Autor: Robert E. Rhoads

Język:

Oprawa: Twarda

Wydawca: Humana Press Inc.

Dostępność: Dostępna u dostawcy

Wysyłamy za 10-13 dni

711.37 zł

This volume§presents detailed laboratory protocols for in§vitro synthesis of mRNA with favorable pro...

Informacje o książce

Autor

Robert E. Rhoads

Język

Angielski

Oprawa

Książka - Twarda

Data wydania

2016

strony

320

EAN

9781493936236

ISBN

1493936239

Enbook ID

02885165

Wydawca

Humana Press Inc.

Waga

7684

Wymiary

178 x 254 x 25

Pełny opis

This volume§presents detailed laboratory protocols for in§vitro synthesis of mRNA with favorable properties, its introduction into§cells by a variety of techniques, and the measurement of physiological and§clinical consequences such as protein replacement and cancer immunotherapy. Synthetic§techniques are described for structural features in mRNA that provide investigational§tools such as fluorescence emission, click chemistry, photo-chemical§crosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced§activation of the innate immune response. Protocols are described for clinical§applications such as large-scale transfection of dendritic cells, production of§GMP-grade mRNA, redirecting T cell specificity, and use of molecular§adjuvants for RNA vaccines. Written§in the highly successful Methods in§Molecular Biology series format, chapters include introductions to their§respective topics, lists of the necessary materials and reagents, step-by-step§laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.§§Synthetic§mRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resource§for both laboratory investigators and clinicians interested in this powerful§and rapidly evolving technology.§§

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Production, Introduction Into Cells, and Physiological Consequences

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