Protein Chromatography

Methods and Protocols

Autor: Sinéad T. Loughran, Dermot Walls

Język:

Oprawa: Miękka

Wydawca: Humana Press Inc.

Dostępność: Dostępna u dostawcy

Wysyłamy za 5-8 dni

487.37 zł

This second edition expands on the previous edition with new chapters that are suitable for newcomer...

Informacje o książce

Autor

Sinéad T. Loughran, Dermot Walls

Język

Angielski

Oprawa

Książka - Miękka

Data wydania

2018

strony

426

EAN

9781493981915

Enbook ID

20091863

Wydawca

Humana Press Inc.

Waga

8134

Wymiary

178 x 254 x 25

Pełny opis

This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.

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