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Plant Virology Protocols

Name: Plant Virology Protocols
Brand: Humana Press Inc.
SKU: 9780896033856
Price: 728.5 PLN
Availability: InStock

From Virus Isolation to Transgenic Resistance

Autor: Gary D. Foster, Sally Taylor

Język:

Oprawa: Twarda

Wydawca: Humana Press Inc.

Dostępność: Dostępna u dostawcy w małych ilościach

Wysyłamy za 13-18 dni

728.50 zł

Plant Virology Protocols offers for the first time a comprehensive collection of state-of-the-art te...

Informacje o książce

Autor

Gary D. Foster, Sally Taylor

Język

Angielski

Oprawa

Książka - Twarda

Data wydania

1998

strony

571

EAN

9780896033856

ISBN

0896033856

Enbook ID

01400699

Wydawca

Humana Press Inc.

Waga

1001

Wymiary

155 x 235 x 32

Pełny opis

Plant Virology Protocols offers for the first time a comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus. The authoritative contributors also discuss the history and mechanisms of coat protein-mediated protection and the key ethical issues surrounding transgenic technology. §Unprecedented in its comprehensiveness and its many time-proven methods, Plant Virology Protocols is certain to become the authoritative standard reference for plant scientists who want to tap the enormous benefits this technology has to offer.A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.

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Plant Virology Protocols

From Virus Isolation to Transgenic Resistance

Informacje o książce

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