Książka Gene Targeting and Embryonic Stem Cells Jim McWhir

Gene Targeting and Embryonic Stem Cells

Język: Angielski
Oprawa: Miękka
Dostępność: 50 % szansa
Przeszukamy cały świat
446.75
Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number...

Informacje o książce

Język
Angielski
Oprawa
Książka - Miękka
Data wydania
2004
strony
216
EAN
9781859963609
ISBN
1859963609
Enbook ID
05120206
Waga
446
Wymiary
174 x 247 x 11

Pełny opis

Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.

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