Gene Knockout Protocols

Name: Gene Knockout Protocols
Brand: Humana Press Inc.
SKU: 9780896035720
Price: 470.91 PLN
Availability: InStock

Autor: Martin J. Tymms, Ismail Kola

Język:

Oprawa: Twarda

Wydawca: Humana Press Inc.

Dostępność: Dostępna u dostawcy w małych ilościach

Wysyłamy za 13-18 dni

470.91 zł

Highly skilled investigators with extensive experience in gene targeting and mouse genetics describe...

Informacje o książce

Autor

Martin J. Tymms, Ismail Kola

Język

Angielski

Oprawa

Książka - Twarda

Data wydania

2001

strony

431

EAN

9780896035720

ISBN

0896035727

Enbook ID

01400771

Wydawca

Humana Press Inc.

Waga

826

Wymiary

152 x 229 x 29

Pełny opis

Highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for the analysis of the mouse phenotype. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today.As the major task of sequencing the human genome nears completion and the full complement of human genes are catalogued, the task of understanding the normal biological functions of genes and how their alteration leads to diseased states becomes more imperative. In Gene Knockout Protocols, highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for genetic phenotype analysis. These proven methods contain step-by-step instructions, as well as notes on pitfalls to avoid, and emphasize techniques that are relevant to researchers carrying out gene targeting work. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. Issues such as the use of existing mouse mutation resources and the influence of genetic background and epigenetic effects upon phenotype are also covered.§State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today.

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