Bacterial Pathogenesis

Methods and Protocols

Autor: Mattias Collin, Pontus Nordenfelt

Język:

Oprawa: Miękka

Wydawca: Humana Press Inc.

Dostępność: Dostępna u dostawcy

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This volume discusses various methods and protocols used for the experimentation of a wide range of...

Informacje o książce

Autor

Mattias Collin, Pontus Nordenfelt

Język

Angielski

Oprawa

Książka - Miękka

Data wydania

2018

strony

357

EAN

9781493982684

Enbook ID

20091903

Wydawca

Humana Press Inc.

Waga

706

Wymiary

178 x 254 x 21

Pełny opis

This volume discusses various methods and protocols used for the experimentation of a wide range of bacterial species, such as Streptococcus pyogenes , Staphylococcus aureus , Streptococcus pneumonia , Listeria monocytogenes , and M ycobacterium marinum . Bacterial Pathogens: Methods and Protocols is divided into 6 parts: Part 1 describes different approaches to identifying and characterizing bacterial effector molecules; Part 2 deals with structural biology of bacterial pathogenesis and how to overcome folding and stability problems with recombinantly expressed proteins; Part 3 details methodology that identifies bacteria in complex communities and how genomes of bacterial pathogens have evolved; Part 4 reflects on the rapid development of advanced imaging techniques that address questions about molecular properties of individual live bacteria, ultrastructure of surfaces, and subcellular localization of bacterial proteins; Part 5 describes methods from in vitro and in vivo modeling of bacterial infections; and Part 6 explores how bacterial pathogens are the true experts of the immune system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Bacterial Pathogens: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.

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