Baculovirus and Insect Cell Expression Protocols

PART I. Introduction

1 Useful Tips, Widely Used Techniques And Quantifying Cell Metabolic Behavior

David W. Murhammer

PART II. Baculovirus Molecular Biology/Development of Recombinant Baculoviruses

2 Introduction to Baculovirus Molecular Biology

Barbara J. Kelly, Linda A. King And Robert D. Possee

3 Baculovirus Transfer Vectors

Robert D. Possee And Linda A. King

4 Recombinant Baculovirus Isolation

Linda A. King, Richard Hitchman And Robert D. Possee

5 Gene Expression In Mammalian Cells Using Bacmam, A Modified Baculovirus System

James A. Fornwald, Quinn Lu, Frederick M. Boyce And Robert S. Ames

PART III. INSECT CELL CULTURE

6 Available Lepidopteran Insect Cell Lines

Dwight E. Lynn and Robert E. Harrison

7 Lepidopteran Insect Cell Line Isolation from Insect Tissue

Dwight E. Lynn

8 Development Of Serum-Free Media For Lepidopteran Insect Cell Lines

Leslie C. L. Chan and Steven Reid

9 Routine Maintenance And Storage Of Lepidopteran Insect Cell Lines And Baculoviruses

Dwight E. Lynn and Robert L. Harrison

PART IV. Protein Production with Recombinant Baculoviruses

10 Small-Scale Production Of Recombinant Proteins Using The Baculovirus Expression Vector System

Jian-Ping Yang

11 Recombinant Protein Production In Large Scale Agitated Bioreactors Using The Baculovirus Expression Vector System

Christine M. Thompson, Johnny Montes, Marc G. Aucoin and Amine A. Kamen

12 Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors

Sue H. Kadwell and Laurie K. Overton

13 Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae

Elena Kovaleva and David C. Davis

14 Production Of Virus-Like Particles For Vaccination

Christine M. Thompson, Marc G. Aucoin and Amine A. Kamen

15 Alternative Strategies for Expressing Multi-Component Protein Complexes in Insect Cells

Stephanie Chen

PART V. Recombinant Protein Production with Transformed Insect Cells

16 Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression

Robert L. Harrison and Donald L. Jarvis

17 Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression

Marija Backovic and Thomas Krey

18 Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression

Robert L. Harrison and Donald L. Jarvis

PART VI Baculovirus Development and Production for Use as Insecticides

19Introduction To The Use Of Baculoviruses As Biological Insecticides

Holly J. R. Popham, Tyasning Nusawardani And Bryony C. Bonning

20 Baculovirus Insecticide Production In Insect Larvae

Nikolai Van Beek and David C. Davis

21Evaluation Of The Insecticidal Efficacy Of Wild Type And Recombinant Baculoviruses

Holly J. R. Popham, Mark R. Ellersieck, Huarong Li and Bryony C. Bonning

PART VII. Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System

22 Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants