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ISBN | 9781493996803 |
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Autor | McManus Jennifer J. |
Wydawca | Humana Pr |
Język | english |
Oprawa | Paperback |
Rok wydania | 2020 |
Liczba stron | 266 |
This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions
Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state
and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.